Histochemical characteristics and distribution of lipofuscin and polyglucosan bodies in the brain of dogs more than 10 years old

The ageing process is accompanied by numerous changes in the brain of dogs, such as accumulation amyloid, fibrosis blood vessel walls and meninges, lipofuscin, presence polyglucosan bodies (PGBs), satellitosis neuronophagia. In this study, lipofuscin PGBs various parts dogs different sexes ages was examined. For purpose, samples were stained using haematoxylin eosin, modified Ziehl Neelsen Periodic acid Schiff (PAS) methods. Lipofuscin visualised PAS methods specific staining on same tissue segments. had accumulated 93% old (more than 10 years old) dog brains, mostly neurons medulla oblongata. percentage age-related pigment other examined segments lower There a small difference lipofuscin-positive individuals between two established method for vast majority (about 93%) old), while not detected group young (up to 5 old). However, occurred all dog?s tissues (for each types, from 90% positive PGBs). results obtained oldest (15 harboured both extracellularly intracellularly, only extracellular seen. large olivary nuclei confirmed more old. This one indications that could be very good animal model studying normal or neurodegenerative diseases.